|
DPS/CPU/04/B1/3006 |
|
08/02/2021 |
|
02/03/2021 14:30:00 |
|
02/03/2021 14:30:00 |
|
02/03/2021 15:00:00 |
|
NA |
|
NA |
|
|
Qualifying Requirement : |
AS IN NIT |
| Sr. no | Item Category | Item Description | Qty | | 1 | CHEMICALS | SUPPLY OF FOLLOWINGS:
PCR Master Mix (2X) , 20 X 100 reactions per kit.
The Master mix should have Taq DNA Polymerase, dNTPs and reaction buffer. | 1 | | 2 | CHEMICALS | Accu Prime Pfx DNA Polymerase, 200 reactions per kit. Should contain-
1. 1 x 100 μL accuprime Pfx DNA Polymerase (2.5 U/µl)
2. 1 x 1 mL 10X accuprime Pfx Reaction Mix
3. 1 x 1 mL 50 mM MgSO4. | 2 | | 3 | CHEMICALS | Top script cDNA Synthesis kit, 100 reactions per kit.
Should contain-
1. Topscript Reverse Transcriptase (200 U/μl)
2. 10X Topscript RT Buffer
3. RNase Inhibitor (40 U/μl)
4. dNTP Mixture (2 mM)
5. Oligo dT (80 μM)
6. Random hexamer (100 μM)
7.Sterile water (RNase free. | 2 | | 4 | CHEMICALS | 1kb DNA Ladder with 13 Blunt-Ended Fragments of 250–10,000 bp at a concentration of 0.1µg/μl | 5 | | 5 | CHEMICALS | Quick Change II XL Site-Directed Mutagenesis Kit, 30 Reactions per kit | 1 | | 6 | CHEMICALS | DpnI, 5,000 units per vial.
20,000 units/ml
Should be supplied with 100% activity in Cut-Smart Buffer and Supplied with 1 vial of Gel Loading Dye(6X) | 1 | | 7 | CHEMICALS | Pre-stained Protein Ladder 500 microliter per vial.
Broad molecular weight (10-245 kDa)
It should have a three-color protein standard with 11-12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa
Proteins should preferably be covalently coupled with a blue chromophore except for (one green and one red band at ~28 kDa and ~80 kDa respectively) when separated on SDS-PAGE(Tris-glycine buffer).
Also to be supplied in a loading buffer for direct loading on gels. | 6 | | | | |
|
|
|
STORES OFFICER, CENTRAL STORES UNIT, DIRECTORATE OF PURCHASE AND STORES, B.A.R.C., TROMBAY, MUMBAI - 400 085. |
|
